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作 者 ：张立平 林伯年 沈德绪

期 刊 ：植物学通报 1998年 15卷 04期 页码：63-67

关键词：葡萄；雌雄异株；性别鉴定；

摘 要 ：通过扫描电镜观察花粉的形态；分析叶片超氧化物歧化酶同工酶(SOD)；RAPD分析基因组DNA多态性，来鉴别葡萄的性别。结果表明：葡萄雌雄株的花粉形态存在着较大的差异；在本实验条件下葡萄雌雄株叶片SOD同工酶谱带一致；一些引物的RAPD产物间存在着多态性，在葡萄雌雄株的基因组DNA序列中存在着碱基排列顺序的差异。实验指出，花粉形态和基因组DNA的多态性分析是进行性别鉴定的有效方法。

Abstract:We differentiated the sexual distinction of dioecism Vitis by observing the shape of pollen under a scanning electron microscope; analyzing the isozyme of SOD in leaves; detecting the polymorphism of genomie DNA with the method of RAPD. The resulsts indicated that:there was a great difference in the pollen shape of male and female Vitis, the pattern of SOD isozyme were the same in male and female dioecism Vitis; some of the results of RAPD were polymorphic between the male and female. The shape of pollen and RAPD were effective for differentiating the sexual distinction of dioecism.

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