The binary vectors which respectively contain chimeric genes ( SCaM1-GFP，SCaM4-GFP ) were constructed and used to transform tobacco ( Nicotiana tabacum L.), pGTV-GFP was used as control. The plasmolyzed cells of transgenic callus treated with CBW were investigated under the laser scanning confocal microscope. Green fluorescence was found in the cell wall of transgenic SCaM1-GFP callus. However there was no green fluorescence in the cell wall of SCaM4-GFP or GFP transgenic callus. These results indicate that SCaM1 can be secreted into the apoplast of plant cells, while SCaM4 does not exist in the apoplast of plant cells.
转SCaM-GFP融合基因烟草中钙调素分泌特性的研究
周华林 马力耕 刘曼 毛国红 孙大业*
（河北师范大学分子细胞生物学实验室，石家庄050016）
关键词： 大豆钙调素亚型；绿色荧光蛋白；分泌
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