In cultivated rice (Oryza sativa L.), F1 pollen sterility is controlled by at least 6 loci of the F1 pollen sterility genes. To map S-b, one of loci, rice variety Taichung 65 (T65) carrying S-bj/S-bj and its near isogenic line TISL2 carrying S-bi/S-bi were used to develop the mapping population. One hundred and fifty-eight microsatellite markers were selected to survey T65 and TISL2. RM13 on chromosome 5 was found to be polymorphic between them. Cosegregation indicated that RM13 was closely linked with locus S-b. Eleven RFLP markers were selected on the corresponding region from the genetic map of Rice Genome Research Program (RGP) of Japan to convert into sequence tagged site (STS) markers. Amplicon length polymorphism (ALP) was carried out, but none of them was found to be polymorphic between T65 and TISL2. Then PCR- based RFLP (PBR) was done using six 4-nucleotide recognizing restriction endonucleases. Polymorphism was detected when PCR products of R830STS and R2213SSTS were digested with TaqⅠ. Genetic analysis indicated that the distance between locus S-b and markers R830STS, RM13 and R2213SSTS were 3.3 cM (centiMorgan), 5.2 cM and 5.5 cM, respectively. These PCR-based markers could be directly used in marker assisted selection. The technical systemcombining genetic mapping and PCR-based marker assisted selection will facilitate the development of molecular breeding.
栽培稻
全 文 ：