The trehalose-6-phosphate synthase gene (TPS) from Saccharomyces cerevisiae Hansen and the drought-responsive promoter from Arabidopsis thaliana (L.) Heynh. have been cloned by PCR procedure. A plant expression vector with TPS under control of Prd29A has been constructed and used for the genetic transformation of tobacco. The transgenic tobacco with Prd29A/TSP demonstrated TPS expression with increased drought tolerance under drought stress. Some obvious morphological changes including dwarf and fine shoot, lancet-shaped leaves and vigorous auxiliary buds have been observed in a few transformed plants.
干旱诱导性启动子驱动的海藻糖-6-磷酸合酶基因载体的构建及转基因烟草的耐旱性
赵恢武 陈杨坚 胡鸢雷 高音 林忠平
（北京大学生命科学学院蛋白质工程及植物基因工程国家重点实验室，北京100871）
摘要：通过PCR程序克隆拟南芥（Arabidopsis thaliana （L.）Heynh．）的干旱诱导性启动子Prd29A及来自酿酒酵母（Saccharomyces cerevisiae Hansen）的海藻糖-6-磷酸合酶基因（TPS），并将它们组成可在植物中表达的载体RT。通过根癌土壤杆菌（Agrobacterium tumefaciens （Smithet-Townsend）Conn）LBA4404介导，获得转基因烟草（Nicotiana tabacum L.）。Southern分析表明，TPS基因已经整合到烟草基因组中。Northern分析表明，TPS基因的表达受干旱胁迫的诱导。转基因烟草的形态发生多种改变，包括植株变矮，茎变细，叶子呈柳叶状，腋芽明显，同时耐旱性得到增强。
关键词： 干旱诱导性启动子；海藻糖-6-磷酸合酶基因；烟草；耐旱性
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