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Establishment of 6VS Telocentric Lines of Haynaldia villosa Resistant to Powdery Mildew Induced by Immature Embryo Culture


The line of T240 6 was selected from 32 SC2 lines of immature embryo culture of T240 (common wheat ( Triticum aestivum L.)× Wheat Haynaldia villosa (L.) Schur. 6D/6V substitution line)through powdery mildew characterization, glutamate oxaloacetate transaminase (GOT) enzyme and gliadin (Gli) analyses and in situ hybridization. All of the individual plants resistant to powdery mildew lacked the locus of GOT at 6V L arm (GOT V2) and had gliadin locus at 6VS arm (Gli-V2) of Haynaldia villosa. All the plants resistant to powdery mildew had one or two telocentric chromosomes that did not pair with wheat chromosomes but paired between themselves. T240-6 was identified as a telocentric line through in situ hybridization. 

幼胚培养创造抗白粉病簇毛麦


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