Abstract:Plant regenerations were achieved from tissue cultures of 31 species of 15 genus of legume plants on A and B media. Various factors including medium composition (major elements, minor element:s, organic components and phytohormones), seed germination rate, illumination and temperature conditions were tested for their effects on callus differentiation in tissue cultures. Combinations of major elements of A or B medium, minor elements and organic components of B5 medium, illumination of 1500–2500 lx, and temperature of 18–27℃ were found suitable for callus differentiation of most legume plants. The calli induced from freshly collected seeds had higher differentiation capacity than from seeds sored for three years. Four types(a,b,c and d) of callus were morphologically distinguished during the differentiation in legume plant tissue cultures. Generally, calli from plants of the same genus belonged to the same type.