Abstract:Esterase isozyme of dry seeds, anthers and immature seeds from 54 accessions of indigenous wheat to China was analysed. The result showed the specialties of esterase isozyme in different tissues. There were 12 zymogram types of esterase isozyme in 54 materials. A special marked band, esterase isozyme band 2A, was found in Xinjiang wheat. Cluster analysis suggested that (1) Yunnan wheat was geneticaly close related to Tibet semi-wild wheat. Both of them are in the same level of evolution and belong to one taxonomical group. (2) Common wheat and Tibet common wheat belong to one group and (3) Xinjiang wheat is a special group, having its own evolutionary process.