Abstract:The electrophoresis analysis of isozymes of Arachis show a very close relationship among four types of cultispecies (A. hypogaea) and A. monticola, the tetraploid wild species is a related species. Among the five diploid wild species of Arachis Section, both A. cardenasii with A genome and A. batizocoi with B genome are found to be relatively nearer to cultis‘pecies than A. correntina, A. stenosperma and A. villosa, while A. rigonii of Erectoides Section and A. pusilla of Triseminala Section are the distant species.