Abstract:A total of 1100 slides containing 45780 airborne pollen grains belonging to 24 families and 37 genera or species were made intermittently for 5 years from 1965 to 1985 Among them there are 9 dominant species with more than 1000 pollen grains in each species. There are 2 peaks of airborne pollens in the atmosphere of Shenyang, one is in Spring (April and May), the other is in Summer and Autumn (July and August). By means of intermittent collection and observation in the past 20 years, it is found that the airborne species and amount of pollen grains are variable, indistinctive, except that Ambrosia L. is significantly. Usually, they vary with the Changes of temperature, national policy for ornamental planting and social hobby. Pollen grains are closely related with allergic diseases. Fluid extracts of 17 species of pollens have been used for skin test and therapy. The fluid extract of Artemisia L. pollen gave the highest rate of positive reaction for skin test, that of the summer-autumn pollens rated the second and that of the spring pollens is the lowest one. Some good results have been obtained by using the fluid extracts of pollens for treatment of allergic diseases.