Abstract:In this paper, the shape and size modification of recent pollen grains have been studied. 63 species of recent pollen grains that belongs to 55 genera, 26 families were observed before and after the acetolysis treatment with LM. The length of polar axis and equatorial axis and the thickness of exine were measured from about 20 pollen grains for each species (the thickness of exine were only measured for acetolysed pollen grains). The significance of shape and size modification was examined with the statistical method "ttest". From this investigati0n it can be concluded that: The pollen modification in size and shape ‘was related to aperture type, pollen size, wall thickness and sculpture depth. Pantoporate, spheroidal pollens and small perporlate pollens usually change little in there size and shape after acetolysis. The size of colpate pollens was generally increased, or their shape changed. The size or shape change usually got bigger as the pollen size, wall thickness and sculpture depth became bigger and bigger. Oblate pollens were apter to change their size and shape than prolate pollens. The P/E ratio of most colpate pollens became a little bigger after acetolysis.