A species of the genus Tupistra，T．pingbianensis J．L．Huang et X．Z．Liu，is described as new，and its karyotype and pollen morphology are reported．  The bimodal karyotype of this species is formulated as 2n＝38＝14m+10sm+8st+6t，and pollen grains are  spheroidal，inaperturate，with rugulate exine sculpture．Based on its floral structure，karyotype and pollen morphology，T．pingbianensis J．L．Huang et X．Z．Liu is considered phylogenetically closely related to T．fungilliformis Wang et Liang．Whether the repense rhizome，solitary leaves of T．pingbianensis J．L．Huang et X．Z．Liu indicate a close relationship between the genus Tupistra and the genus Aspidistra needs further studies．