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Resveratrol production in suspension cell cultures of Vitis vinifera

葡萄细胞悬浮培养生产白藜芦醇



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ChineseJournalofBioprocessEngineering
Vol.11No.5
Sep.2013
doi:10.3969/j.issn.1672-3678.2013.05.004
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ResveratrolproductioninsuspensioncelculturesofVitisvinifera
LONGXiaofeng1,ZHENGLiping2,ZHAOPeifei3,WANGJianwen1
(1.ColegeofPharmaceuticalSciences,SoochowUniversity,Suzhou215123,China;
2.JintanglangSchoolofArchitectureandUrbanEnvironment,SoochowUniversity,Suzhou215123,China;
3.InstituteofHorticulturalCrops,YunnanAcademyofAgriculturalSciences,Kunming650205,China)
Abstract:ThecaliwereinducedfrompeelofVitisviniferacv.KyohoonB5mediumsupplementedwith
20mg/L6BAand01mg/L2,4D.Thesuspensionculturesystemwasthenestablishedwithinitial
calusinoculationof50g/LinliquidB5mediumwith10mg/L6BAand005mg/L2,4Dfor
resveratrolproduction.After18dshakercultivation(120130r/min)at2527℃ underdarkness,the
biomassandresveratrolproductionintheculturereachedpeaklevelsof1617g/Land9569μg/gDW.
Theresveratrolproductioninthecelsreached23573μg/gDW after6dtreatmentwithmethyl
jasmonateat100μmol/Ltothe12dsuspensioncultures.
Keywords:Vitisvinifera;suspensioncels;resveratrol;methyljasmonate
  
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