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作 者 ：南宫自艳,高宝嘉*,杨君

期 刊 ：生态学报 2009年 29卷 4期 页码：1661~1667

关键词：松毛虫属；等位酶变异；亲缘关系；

Keywords:Dendrolimus, allozyme variation, phylogenetic relationship,

摘 要 ：采用等位酶聚丙烯酰胺凝胶电泳技术对松毛虫属5个种和亚种的野生种群进行了亲缘关系和遗传变异的研究。8种等位酶系统(乳酸脱氢酶LDH、苹果酸脱氢酶MDH、苹果酸酶ME、乙醇脱氢酶ADH、甲酸脱氢酶FDH、谷氨酸脱氢酶GDH、过氧化物酶POD、过氧化氢酶CAT)共检测到12个基因位点，其中6个位点为多态位点，检测到15个等位基因。松毛虫属5个种和亚种的总体水平多态位点比率P＝50%，平均有效基因数A = 1.917，平均期望杂合度He = 0.267，平均遗传距离为0.0730～0.5701。遗传参数表明松毛虫属昆虫种间存在较高程度的遗传变异，聚类图和遗传距离数据表明赤松毛虫与马尾松毛虫亲缘关系最近，落叶松毛虫与思茅松毛虫亲缘关系最远。

Abstract:The genetic diversity among five species of Dendrolimus, i.e.Dendrolimus punctatus punctatus Walker, D. punctatus spectabilis Butler, D. superans Butler, D. houi Lajonquiere and D. kikuchii Matsumura was investigated using polyacrylamide gel electrophoresis. Eight allozymes, such as Lactate dehydrogenase (LDH), Malate dehydrogenase (MDH), Malic enzyme (ME), Alcohol dehydrogenase (ADH), Formic acid dehydrogenase (FDH), Glutamate dehydrogenase (GDH), Catalase (CAT) and Peroxidase(POD) were analyzed. Twelve loci and fifteen alleles were detected, among which six loci were polymorphic (P = 50.0%). The average number of alleles per locus A was 1.917, and the expected heterozygosity He was 0.267, and their mean genetic distance was 0.0730-0.5701. The results indicated that the genetic diversity of Dendrolimus was high among the five species. According to the hereditary parameters of the five species, D. punctatus punctatus Walker and D. punctatus spectabilis Butler had the closest genetic relationship, while D. superans Butler and D. kikuchii Matsumura had the most distant genetic relationship.

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