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作 者 ：刘新春,吴成强,张昱,杨敏,李红岩

期 刊 ：生态学报 2005年 25卷 4期 页码：842~847

关键词：PCR-DGGE方法；活性污泥系统；微生物群落分析；

Keywords:activated sludge system, community structure, PCR-DGGE,

摘 要 ：应用PCR- DGGE方法,对在相同的操作条件下分别用低温菌和常温菌接种的两套活性污泥系统中的微生物群落结构的动态变化进行了追踪。研究结果表明:由于工艺和操作条件相同,两系统的微生物群落结构的相似性随着运行时间的增加而增加。PCR- DGGE方法可以在一定程度上反应出系统以及操作条件对微生物群落结构变化的影响。

Abstract:Population dynamics in two activated sludge systems inoculated with seed sludge cultivated respectively at a low temperature ((5±1)℃) and normal temperature ((20±1)℃) were followed under the same operation conditions using the PCR-DGGE method. The genomic DNA was extracted from activated sludge using phenol-chloroform. PCR amplification of the extracted microbial 16S rRNA gene fragments was performed using primers GC341F and 518R. The result of agarose gel (1.8%) electrophoresis shows that the PCR product...

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