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作 者 ：王小纯,熊淑萍,马新明,张娟娟,王志强

期 刊 ：生态学报 2005年 25卷 4期 页码：802~807

关键词：氮素形态；专用小麦；硝酸还原酶；谷氨酰胺合成酶；谷氨酸合酶；蛋白质含量；

Keywords:nitrogen forms, wheat cultivars with specialized end-uses, nitrate reductase, glutamine synthetase, glutamate synthase, protein content,

摘 要 ：采用盆栽方法研究了氮素形态对不同专用型小麦开花后氮素同化关键酶活性及籽粒蛋白质含量的影响。结果表明:不同专用型小麦氮素同化关键酶硝酸还原酶、谷氨酰胺合成酶和谷氨酸合酶对氮素形态的反应不同。强筋小麦豫麦34施用酰胺态氮对旗叶硝酸还原酶和谷氨酰胺合成酶活性、籽粒谷氨酰胺合成酶和谷氨酸合酶活性具有明显的促进作用,最终籽粒蛋白质含量较高;中筋小麦豫麦4 9在施用铵态氮时,3种氮素同化关键酶活性均有较大增强,籽粒蛋白质含量最高;弱筋小麦豫麦5 0硝酸还原酶活性以铵态氮处理最高,而籽粒和旗叶谷氨酰胺合成酶和谷氨酸合酶活性在酰胺态氮处理下明显增强,酰胺态氮对籽粒中蛋白质含量的增加具有明显的促进作用。相关性分析表明,籽粒蛋白质含量与旗叶GS活性和籽粒GOGAT活性呈显著或极显著正相关,与旗叶NR活性和GS活性、籽粒GOGAT活性相关性不显著。

Abstract:In order to understand the effects of nitrogen form on key enzyme activity involved in nitrogen metabolism and grain protein content of speciality wheat cultivars, pot experiments were carried out at experimental station of Henan Agricultural University during 2000～2002. Soil containing 9.8 g/kg organic matter, 0.986 g/kg total N, 25.43 mg/kg olsen-p and 259 mg/kg NH_4OAc-K was used in the experiments. 18kg of sieved soil was placed in each 30cm×40cm pot. Three cultivars were used in experiments including Y...

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