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作 者 ：潘慧云,李小路,徐小花,高士祥*

期 刊 ：生态学报 2008年 28卷 12期 页码：6159~6164

关键词：烯效唑；伊乐藻；氧化胁迫；氧化损伤；

Keywords:uniconazole, Elodea nuttallii, oxidative damage, oxidative,

摘 要 ：研究了烯效唑对沉水植物伊乐藻生长及抗氧化酶活性的影响。结果表明，烯效唑可以刺激伊乐藻新芽萌发，新生枝条节间距减小，叶片紧密，同时烯效唑对成熟枝条的生长具有明显的抑制作用。低浓度（≤1.0mg/L）、短时间的烯效唑暴露可促进叶绿素a含量的增加，随着暴露时间的延长，处理组叶绿素a含量降低，叶绿素b含量显著增加。烯效唑胁迫下，伊乐藻体内3种抗氧化酶反应灵敏，SOD活性受到显著诱导，CAT活性先升高后降低，POD活性先升高后降低后又升高。说明烯效唑可对植物产生氧化胁迫，诱导抗氧化酶活性升高，当胁迫超过一定强度时，活性氧不能及时清除，对植物体产生氧化损伤。

Abstract:Effects of uniconazole on the growth and activitives of anti-oxidation enzymes of Elodea nuttallii were studied. The results showed that uniconazole could stimulate the sprout bourgeoning but restrain the growth of frond remarkably. The internode length of the new branches was smaller and leaves grew closely. Low concentrations of uniconazole increased the content of chlorophyll a at the beginning of the exposure, and, at longer exposure time, chlorophyll a content decreased, but chlorophyll b content increased greatly. Anti-oxidation enzymes of Elodea nuttallii were sensitive to the exposure of uniconazole. The activities of SOD (superoxide dismutase) were greatly induced, and the activities of CAT (catalase) increased first and then decreased. The activities of POD (peroxidase) increased at the beginning, then decreased, and then increased again. The results revealed that uniconazole could result in oxidative stress to Elodea nuttallii, and induce elevated activities of anti-oxidation enzymes. When stress intensity exceeded a certain value, reactive oxygen species could not be removed in time and would result in oxidative damages to the plant.

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