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作 者 ：崔继哲,祖元刚,聂江城

期 刊 ：生态学报 2002年 22卷 7期 页码：982~989

关键词：羊草；RAPD；遗传多样性；遗传分化；

Keywords:Leymus chinensis, RAPD, genetic diversity, genetic differentiation,

摘 要 ：对松嫩草原上分布的灰绿型和黄绿型羊草 9个种群进行了 1 5个引物的 RAPD分析。统计结果表明 ,两类种群的扩增片段数和多态位点比率明显不同 ,黄绿型种群低于灰绿型 ,其值分别 <90与 >1 0 0 ,<5 0 %与 >70 %。比较了 7种不同统计方法据 RAPD表型或基因型频率估算的种群遗传多样性 ,几种统计结果都揭示 ,黄绿型种群低于灰绿型种群。用 FIS值矫正种群对 Hardy-Weinberg平衡的偏离后 ,估算等位基因频率 ,通过 Shannon指数和 Nei指数估计羊草种群间分化分别为 37.6 %和 35 .7% ,高于等位酶的分析。讨论比较了等位酶和 RAPD分析结果的异同。

Abstract:Leymus chinensis (Trin.)Tzvel., which is one of rhizome grass species, has two ecotypes in the Songnen Plain,including a gray green type and a yellow green type. The levels of genetic diversity and genetic differentiation based on RAPDs were assessed among seven populations of the gray green type and two populations of the yellow green type (a total of 105 individuals). Fifteen 10-mer primers screened from seventy Operon‘s primer series were used to amplify RAPD fragments using a standard RAPD protocol. The...

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