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作 者 ：张洪勋,王晓谊,齐鸿雁

期 刊 ：生态学报 2003年 23卷 5期 页码：988~995

关键词：微生物生态；荧光标记蛋白；FISH；PCR-RFLP；PCR-SSCP；PCR-DGGE；PLFA；

Keywords:vegetation dynamics model, human activities, coupled differential equations, vegetation cover, rate of soil erosion,

摘 要 ：微生物培养及显微技术作为鉴定微生物种群的手段有很大的局限性 ,因为环境中大多数微生物处于“存活但不能培养”的状态。因此 ,不依赖于微生物培养的生物化学以及分子生物学方法正被广泛地用于微生物生态学研究。主要介绍了荧光技术 ,基于 PCR的分析技术和 PLFA等技术在表征微生物多样性研究中的某些进展。

Abstract:The cultivation of microbes and microscopy techniques as a means to identify microbial communities have severe limitations, since the majority of microbes in the environment are viable but nonculturable. For this reason, the methods of biochemistry and molecular biology that are independent of culturing are being widely used in the studies of microbial ecology. In this paper, fluorescent technique, PCR-based technique, PLFA technique and others to characterize diversity of microorganisms are reviewed. ...

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