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作 者 ：方智振， 叶新福， 周丹蓉， 潘少霖， 姜翠翠

期 刊 ：核农学报 2016年 30卷 2期 页码：209-215

关键词：李；辐射；红肉；ISSR分析；

Keywords:plum, radiation, red-flesh, ISSR analysis,

摘 要 ：本研究通过⁶⁰Co-γ辐射诱变,获得1个三月李红肉迟熟突变体,为了确定突变体的DNA变异情况,采用ISSR分子标记技术进行检测。从22条引物中筛选出14条多态性较好的引物对三月李、表型未发生显著变异的诱变材料及红肉迟熟突变体进行遗传差异分析。结果表明,共有8条ISSR引物可检测到三月李及其突变体在分子水平上的差异。因此,ISSR标记能较好地区分辐射诱变获得的李突变材料,可为李辐射诱变育种的早期鉴定提供参考。

Abstract:In this study, a red-flesh and late-ripening mutant of Sanyue plum was obtained via ⁶⁰Co-γ ray radiation. ISSR molecular marker technique was employed to study the variation of the mutant at DNA level. 14 primers with polymorphism selected from 22 primers were used to analyze the genetic differences among Sanyue plum, mutagenic materials had no significant variations with the mutant. The results indicated differences between the parent and the mutant could be detected with eight primers on the molecular level. The results propose that plum mutants obtained by irradiation can be distinguished by ISSR markers. The results could offer the references for early identification of radiation mutation breeding in plum.

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