---

作 者 ：张豆豆， 梁新华， 王俊

期 刊 ：核农学报 2016年 30卷 1期 页码：28-34

关键词：甘草种子；水浸液；萌发；基因表达；实时荧光PCR；化感；自毒作用；

Keywords:Glycyrrhiza uralensis Fisch., aqueous extracts, germination, gene expression, real-time PCR, allelopathy, auto toxictity,

摘 要 ：为探讨甘草种子是否存在化感自毒作用,以甘草种子为试验材料,采用常规萌发试验和实时荧光定量PCR方法,研究10g·L^-1浓度的甘草种子水浸液对其种子萌发率、GuSQS1基因和GubAS基因表达的影响。结果表明:甘草种子具有较强的化感自毒作用。10g·L^-1 甘草种子水浸液处理下甘草种子的萌发率为62%,极显著低于对照处理(76%);该处理极显著抑制了GubAS基因的表达,但对GuSQS1基因的表达却具有显著促进作用。该研究结果可为人工调控栽培甘草质量提供一定参考。

Abstract:To characterize the autointoxication potential of Glycyrrhiza uralensis Fisch., the influences of Aqueous extract from seeds of G. uralensis at a concentration of 10g·L^-1 on germination and relative expression of GuSQS1 and GubAS were studied. The results indicated that the germination rate of G. uralensis seeds in the aqueous extract was 62%, which is significantly lower than the germination rate of 76% in the pure water treatment. The aqueous extract treatment also significantly inhibited the GubAS gene expression. In contrast, the aqueous extract promoted the relative expression of the GuSQS1 gene. Therefore, it was concluded that the seed aqueous extract of G. uralensis can inhibit its seed germination and have significant but distinct effects on the expression of two key genes involved in the bio-synthesis pathway of glycyrrhizic acid. The findings of high autointoxication of G. uralensis seeds should be valuable for agronomic production of the species.

---