Abstract:Distant hybridization is known as an important protocol for creation new germplasms and improvements of cultivars in ornamental plants. However, false hybrids were regularly obtained in breeding practices. Thus, it is important to identify the hybridity of the putative progenies for selecting true hybrids in ornamental plants distant hybridization. In this paper, the causes for the production of false hybrids were briefly introduced, and the main methods about hybridity identification, including morphological characteristics, cytological genetics, molecular cytogenetics, molecular markers, and physiological and biochemical indexes, were comprehensively summarized. The identification methods were reviewed with an emphasis on in situ hybridization technologies, including FISH and GISH, in addition to molecular markers, such as RAPD, AFLP and SSR. Moreover, the hybridity identification strategy and future research directions in this field were suggested and prospected, for the purpose of providing a reference for relative researches.