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期 刊 ：核农学报 2009年 23卷 6期 页码：1060-1064

关键词：Hg2+；Cr3+和Pb2+胁迫；绿豆；膜质过氧化物；抗氧化酶活性；

Keywords:Hg2+、Cr3+andPb2+stress, mungbean, themembraneperoxidation, antioxidantenzymesactivity,

摘 要 ：研究了不同浓度Hg²⁺、Cr³⁺ 和Pb²⁺胁迫条件下，绿豆花荚期叶片中膜脂过氧化物（MDA）含量及抗氧化酶，包括(超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT))活性的变化情况。结果表明，Hg²⁺、Cr³⁺和Pb²⁺胁迫后，随着浓度的升高，绿豆叶片中MDA含量也逐渐升高，与对照相比均呈差异显著性；在Cr³⁺和Pb²⁺胁迫下，绿豆叶片中SOD酶活性随着浓度的升高呈现升高的趋势；在低、中浓度Hg²⁺胁迫下，SOD酶活性低于对照，但高浓度下，SOD酶活性高于对照；Hg²⁺处理后，POD随着浓度的增加，表现出先升高后降低又升高的趋势；低、中浓度Cr ³⁺处理使绿豆叶片内POD活性降低，但在高浓度时表现出POD活性增强；随着Pb²⁺处理浓度的增加，POD活性逐渐降低；Hg²⁺ 和Cr³⁺处理使CAT活性升高，但Pb²⁺处理后，CAT活性随浓度的升高先下降，再升高，然后又下降。研究表明Pb²⁺对绿豆的生态毒性较大。

Abstract:The effects of different concentration of Hg2+，Cr3+ and Pb2+ on the membrane peroxidation (MDA) content and the activities of antioxidant enzymes(superoxide SOD, POD, CAT in mung bean in podding period were studied. The results showed that, under stress of Hg2+,Cr3+and Pb2+, the MDA content of leaves in mung bean increased gradually, with the increase of treating concentration, it shown a significant difference compared with control. The activity of SOD increased as the concentration increased under the stress of Cr3+ and Pb2+. The activity of SOD was lower than control under the low concentration(10mg/kg-soil) and moderate concentration(50mg/kg-soil) of Hg2+, but it was higher than control with high concentration of Hg2+. With the increase of concentration under the stress of Hg2+, the POD activity was first increased, then decreased and increased again. The POD activity decreased under the stress of low concentration and moderate concentration of Cr3+, while the it increased under the high concentration of Cr3+. As the increase of Pb2+concentration, the POD activity decreased gradually. The CAT activity increased under treatment of Hg2+ and Cr3+, but with the increase of treating concentration the CAT activity was in the partten of decrease-increase-decrease under the treatment of Pb2+. This investigation indicated that Pb2+ had aseriously ecotoxicological effect on mung bean.

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