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作 者 ：李美茹， 刘鸿先， 王以柔

期 刊 ：热带亚热带植物学报 1999年 7卷 4期 页码：323~328

关键词：氧化胁迫；水稻幼苗；冷适应性；抗氧化酶；

Keywords:Oxidative stress, Rice seedlings, Cold tolerance, Antioxidatase,

摘 要 ：利用H₂O₂和甲基紫精（MV）对水稻幼苗作三种不同程度的氧化胁迫预处理。结果表明：轻度氧化胁迫预处理（10 μmol/L H₂O₂或10 μmol/L MV处理４ｈ）提高了水稻幼苗的抗冷力，严重氧化胁迫预处理（10 μmol/L H₂O₂或10 μmol/L MV分别处理16 h和40 h）则削弱水稻幼苗的抗冷力。氧化胁迫预处理刺激了水稻幼苗叶片抗氧化酶（SOD，CAT，POX和APX）的活性。经冷胁迫后，不同预处理苗的叶片抗氧化酶活性、膜脂过氧化和膜结构的变化趋势不同：轻度氧化胁迫预处理使幼苗仍保持较高的抗氧化酶活性，减轻了由冷胁迫引起的膜脂过氧化和细胞膜的渗漏程度，而严重氧化胁迫预处理则相反。因此，水稻幼苗对氧化胁迫感知并作出反应的机制（氧化应激机制）在水稻幼苗对低温反应和适应过程中起着很重要的调节作用。

Abstract:Effects of different extents of oxidative stress caused by 10 μmol/L H₂O₂ or μmol/L methyl viologen (MV) on cold tolerance of rice seedlings were studied. Theresults showed that mild oxidative stress (10 μmol/L H₂O₂ or 10 μmol/L MV for 4 h)could induce cold tolerance, while severe oxidative stress might aggravated chilling injuryon rice seedlings. The antioxidatase (POX, SOD, CAT andf APX) activities in leaves fromvarious pretreated seedlings were all increased. After chilling stress, the changes in antioxidatase activities．electrolyte leakage and MDA content among different pretreated seedlings were difierent Mlid oxidative stress could sustain high IeveI of antioxidatase activities，reduce the increase of membrane lipid peroxidation and cellular electrolyte leakage induced by chilling stress； but contrary effect was observed by severe oxidative pretreatment. It is suggested that the mechanism of rice seedlings responsing to oxidative stress (cellular oxidative stress response) might be the important regulatory mechanism for rice seedlings during chilling stress or cold tolerance.

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