Abstract:The changes of anther proteins in a typical Hubei Photoperiod-sensitive genie male -sterile rice (HPGMR) Nongken 58s under the induction of long day (14/10h, light/dark) and short day (9/15h, lisht/dark) were analysed by two-dimensional polyacrylamide gel electrophoresis. Proteins from anthers were resolved into 180 components.The protein electrophoretogram showed that there were evident difference between sterile anthers and fertile anthers at diffent stages of pollen development. Four of the protein spots, namely P1 (70KD, pI6. 2), P2 (68KD,pI6. 2), P3 (37KD, pI7. 4) and P7 (38KD, pI6. 2) were oborved in sterile anthers from early uninucleate stage to trinucleate stage of pollen. These proteins were considered as the specific proteins related to sterility formation under iong day induction.Free histone was also analysed by SDS-PAGE in anthers. It showed that the histone patterns were chansed with the development of pollen, and the relative contentS of free histone in sterile anther were lower than that in fertile anther by 19. 6% and 7. 4% at the uni- and dinucleate stages of pollen. The decrease of Part of flee histone content may be caused by the effects of decreasing amount of synthetic DNA in the sterile anthers.