Abstract:A procedure for isolation,culture and plantlet regeneration from mesophyll protoplasts of Solanum quitoense Lain.was reported. Young leaf protoplasts from aseptic plants grown in controlled environment were plated at 1×104 protoplasts ml-1 on modified K8p medium with 2,4-D 0.5mg L-1+NAA 1mg L-1+BA 0.5mg L-1. Protoplast division was initiated after 3 days, and one week later the 3rd and 4th divisions were observed. After culture for four weeks the protoplast-derived calli grow to a size of 1─ mm in diameter, and the planting effeciency (PE) amounted to 0.1─0.2%. Protoplast-derived calli were transferred onto MS+2,4-D 0.5mg L-1 for proliferation. One month later the calli were transferred again onto MS+IAA+BA (or ZT). Subsequently, shoots occured at 42.9% effeciency on MS+IAA 1mg L-1 +ZT 5.0mg L-1. Shoots were rooted on MS +IAA 0.2mg L-1 successfully.