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作 者 ：张安世;刘莹;张为民;赵利新

期 刊 ：植物研究 2014年 34卷 5期 页码：712-715

关键词：太行菊；SRAP；均匀设计；优化；

Keywords:Opisthopappus taihangensis, SRAP, uniform design, optimization,

摘 要 ：为探索适宜太行菊的SRAP-PCR反应体系，采用两轮均匀设计试验，对SRAP-PCR反应体系中模板DNA、引物和2×Taq MasterMix的用量进行优化。结果表明：在10 μL SRAP-PCR反应体系中，含模板DNA 1.3 μL，正反引物0.35 μL，2×Taq MasterMix 5.3 μL。在此最佳条件下，利用Me1/em4引物对11株太行菊扩增的条带清晰、多态性好，表明此反应条件适合于太行菊的SRAP-PCR反应体系。

Abstract:In the present study, SRAP-PCR reaction conditions for Opisthopappus taihangensis(Ling) Shih were optimized. The factors affecting SRAP results of O.taihangensis were investigated by uniform designs of two times. The optimal protocol was accomplished in 10 μL reaction volumes containing 1.3 μL template DNA, 0.35 μL primers and 5.3 μL 2×Taq MasterMix. Under this optimal condition, clear and polymorphic bands from 11 O.taihangensis plants had been obtained using Me1/em4 primers. The result showed that the reaction condition was suitable for SRAP-PCR reaction system of O.taihangensis.

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