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作 者 ：刘德团;李婉莎;查宏光;杨永平;胡向阳*

期 刊 ：植物研究 2011年 31卷 4期 页码：414-421

关键词：高山植物；胁迫；抑制消减杂交；

Keywords:alpine plant, stress, suppression subtractive hybridization(SSH),

摘 要 ：应用磁珠介导的抑制消减杂交方法，以青藏高原白马雪山流石滩地区植物拟耧斗菜为实验材料，分别在白天与夜晚采样，以白天样品为目标，晚上样品为驱动样品，分离昼夜差异表达的基因。随机挑选了96个差异ESTs克隆并进行测序，测序结果进行blastx网上数据库比对、Blast2go功能注释和KEGG代谢路径分析。结果表明：绝大部分ESTs功能与物质能量代谢、生物合成、蛋白和核酸结合、转移酶活性相关，还有少数与逆境胁迫相关等。这为进一步研究高山植物逆境适应的分子机制奠定了基础。

Abstract:Suppression subtractive hybridization (SSH) based on magnetic beads technology was used to screen day-night differential expression genes of Paraquilegia microphylla living on the scree of the Baima snow mountain of Tibetan plateau. The daytime sample was used as tester and nighttime sample as driver for further analysis. Finally, 96 differential ESTs between two samples were sequenced. Sequences were analyzed with BlastX, Blast2go and KEGG. The results indicated that most differential expression proteins are related to material and energy metabolism, biosynthetic process, have nucleic acid binding, protein binding and transferase activity; and a few are responsive to stress, etc. These results will provide some prerequisites for further researches on alpine plants under harsh environments.

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