Abstract:By taking samples to dispose artificial low-temperature gradient freeze,crossing winter naturally without protecting,freeze and melt season in spring and autumn,testing the physiology targets changing regularity of materials’ cold-resistant,researching the cold-resistant mechanism and ability. Significance analysises through Excel and SAS data software indicate that soluble carbohydrate and protein content and conductivity rate are unimodal curvilinear motion with the reducing of temperature,reach supreme value in winter and the artificial most low temperature; POD keeps higher enzyme activity in autumn and spring,especially the enzyme activity is the highest in spring;the injury of unimodal curve to materials in spring is stronger than autumn; Parthenocissus quinquefolia’s semilethal temperature is -26.89℃，Parthenocissus tricuspidata’s semilethal temperature is -24.07℃，Ability of the cold-resistant and crossing winter without protecting of Parthenocissus quinquefolia is stronger than Parthenocissus tricuspidata; Experiment materials have certain ability of surviving the winter among Harbin’s cold environment; The growth behaves after the experiment material survives the winter revealing conforms with the experiment conclusion.