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作 者 ：严一字;吴基日*

期 刊 ：植物研究 2007年 27卷 3期 页码：308-312

关键词：桔梗；RAPD；亲缘关系；

Keywords:Platycodon grandiflorum DC., RAPD, genetic relationship,

摘 要 ：对取自中国、日本、韩国和朝鲜等东亚地区的24个桔梗种质资源，利用RAPD-PCR标记方法，分析了其遗传变异和遗传关系，旨在为桔梗的品种鉴定和杂交育种的亲本选择提供理论依据。结果表明： （1）用11个引物扩增出131条谱带，平均每个引物扩增出11.9条谱带，并扩增出61个多态性谱带，平均每个引物扩增出5.5条多态性谱带，显示出了相对高的多态率（46.6%）。（2）得到的RAPD数据的遗传相似性范围为0.668～0.994，并以遗传相似系数0.78为标准，将24个桔梗种质资源分为7个类群。

Abstract:The genetic variation and genetic relationships among 24 samples of Platycodon grandiflorum DC. cultivars collected from East-Asian area (China, Japan, Korea and DPRK)were analyzed by the method of RAPD-PCR markers. The results showed that 131 bands were amplified by 11 primers with an average of 11.9 bands per primer and 61 polymorphic bands were obtained with an average of 5.5 bands per primer, which revealed relatively high percentage of polymorphic bands (46.6%). The genetic similarity calculated from RAPD data varied from 0.668 to 0.994, and the 24 samples of Platycodon grandiflorum DC. cultivars were divided into 7 major groups on a criterion of 0.78 coefficient.

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