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作 者 ：郑桂灵;李鹏

期 刊 ：植物研究 2011年 31卷 (3)期 页码：313-317

关键词：AAPT1基因；RNAi；植物表达载体；

Keywords:AAPT1 gene, RNAi, plant transformation vector,

摘 要 ：以拟南芥中氨基乙醇磷酸转移酶基因AAPT1作为RNAi的靶向序列，采用RT-PCR的方法获得目的DNA片段。然后以pBS-T-AAPT1载体为基础,构建了由35s启动子调控的AAPT1基因RNAi的植物表达载体pART27-AAPT1(1,2),并通过电击法将重组质粒导入根癌农杆菌C58中。AtAAPT1基因RNAi的植物表达载体的构建对于研究AAPT1基因的功能和应用具有重要的理论价值。

Abstract:Aminoalcoholphosphotransferase gene AAPT1 from Arabidopsis thaliana was used as target sequence for RNA interference in this paper. The target sequence was linked to pBS-T cloning vector after obtained by RT-PCR reaction. A plant transformation vector pART27-AAPT1(1,2) was constructed from pBS-T, including AAPT1 gene controlled by CaMV35s promoter in this experiment. Then the recombination plasmid was transfected into Agrobacterium C58 by electroporation-mediated way.

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