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期 刊 ：植物保护学报 2010年

关键词：内生枯草芽孢杆菌；香蕉组织培养；香蕉枯萎病菌4号生理小种；保护酶活性；

Keywords:endophytic Bacillus subtilis, banana tissue culture, Fusarium oxysporum f.sp.cubenserace 4(FOC), defense enzymes activities,

摘 要 ：为探讨香蕉内生枯草芽孢杆菌EBT1对香蕉生长和抗病性的诱导作用,研究了EBT1培养液对台蕉2号(AAA)丛生芽增殖及再生苗生长的影响,并测定香蕉苗对枯萎病4号生理小种孢子及其毒素的抗性。结果表明,培养基中添加10%(v/v)EBT1培养液能明显提高丛生芽的增殖、苗株重、假茎高、抗毒素和抗病能力,并增加香蕉苗保护酶SOD、PAL、POD、PPO和CAT活性。在EBT1培养液全程诱导、增殖诱导和生根诱导中,全程诱导对香蕉苗生长及其抗性的诱导效果最好,增殖诱导次之,毒素处理1～7天后,源于EBT1培养液全程诱导处理的香蕉苗酶活水平较高,其5种保护酶活性峰值分别为对照的3.82、1.18、1.50、2.70和1.52倍。

Abstract:In order to study the inducing effects of endophytic Bacillus subtilis strain EBT1 isolated from the banana on the growth and resistance to wilt disease caused by Fusarium oxysporum f.sp.cubense race 4 (FOC) in banana, the effects of the EBT1 culture filtrates on the buds multiplication and regenerated plantlets growth of ‘Taijiao 2’ banana (Musa spp., AAA) were investigated, and the resistance of plantlets to conidia and toxin of FOC was determined. The results showed that the additive 10% (v/v) EBT1 culture filtrate to medium could obviously increase the buds multiplication, plantlets weigh, pseudo-stem height, and resistance of plantlets to conidia and toxin of FOC with the enzymes activities increased for SOD, PAL, POD, PPO, CAT in plantlets. Among the whole course inducement (WCI), multiplication course inducement(MCI)and radication course inducement (RCI) with EBT1 culture filtrates, the WCI showed the best inducement effect on the increasing growth and resistance of plantlets, followed by MCI. The high enzymes activities was observed in plantlets from WCI after 1 d to 7 d treated with toxin, the maximum peaks of SOD, PAL, POD, PPO and CAT activities were 3.82, 1.18, 1.50, 2.70 and 1.52 times control respectively.

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