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期 刊 ：植物保护学报 2011年

关键词：植物内生放线菌Lj20；抗真菌物质；灰霉病；病害防治；

Keywords:endophytic actinomycete Lj20, antifungal substance, Botrytis cinerea, disease control,

摘 要 ：菌株Lj20是从健康辣椒植株分离得到的一株有抗真菌活性的植物内生放线菌,为了进一步明确其生物防治潜力,利用大孔吸附树脂、离心薄层层析以及柱层析等技术对Lj20代谢产物中的抗真菌活性物质进行了分离和提纯,并采用生长速率法测定了其代谢产物对黄瓜灰霉病菌的抑制作用,同时在温室中对Lj20发酵液粗提物的防病效果进行了试验。从Lj20代谢产物中分离得到一个抗真菌活性物质,该化合物对黄瓜灰霉病菌具有较强抑制作用,EC₅₀值为174.94 mg/L;500 mg/L Lj20发酵液粗提物对黄瓜灰霉病的防治效果达62.5%;经鉴定其化合物结构为3, 5-二叔丁基-4-羟基-苄基甲醚。结果表明,放线菌Lj20有进一步开发和利用的价值。

Abstract:Endophytic actinomycete Lj20 with antifungal activity was isolated from healthy capsicum plants. In order to determine its potential application in biological control, the antifungal activity substance was separated and purified from metabolites of Lj20 by the means of macroporous absorbing resin, centrifugal thin layer chromatography and column chromatography. The inhibitory efficacy of Lj20 metabolites on Botrytis cinerea Pers was also studied by means of the inhibition rate of mycelium growth in vitro and the control efficacy in the greenhouse. The results showed that the structure of a compound isolated from the Lj20 metabolites was identified as 3, 5-di-tert-butyl-4-hydroxybenzyl methyl ether by analysis of IR (infrared spectroscopy) and ¹H-NMR (proton nuclear magnetic resonance spectroscopy) spectroscopic data. This compound indicated the inhibition efficacy on B.cinerea in vitro with the EC₅₀ was 174.94 mg/L. In addition, the control efficacy of crude extracts from Lj20 fermentation on B.cinerea reached 62.5% at the concentration of 500 mg/L in the greenhouse experiments, indicating that this endophytic actinomycete Lj20 had a potential value for further exploitation and utilization.

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