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期 刊 ：植物保护学报 2011年

关键词：韭菜迟眼蕈蚊；标准糖醋酒液；诱杀；气相色谱与质谱联用；气相色谱与触角电位仪联用技术；

Keywords:Bradysia odoriphaga, sugar-acetic acid-ethanol-water solutions, trapping effects, GC-MS, GC-EAD,

摘 要 ：为了明确糖醋酒液诱集韭菜迟眼蕈蚊的作用机制,比较了4种糖醋酒液配方对该虫的田间诱杀效果,并采用动态顶空吸附法、气相色谱与质谱联用技术(GC-MS)、气相色谱及触角电位仪联用技术(GC-EAD)测定了最优配比对该虫的电生理反应.绵白糖、乙酸、无水乙醇及自来水的比例为3:3:1:80的糖醋酒液A为最优配比,该配比与其它各处理诱虫效果差异显著(P<0.05).糖醋酒液A的挥发物有33种,其中酮类化合物7种、醛类化合物8种、醇类化合物2种、烷烃类化合物12种、酯类化合物3种、酚类化合物1种,但4种配比液的挥发性成分之间差异较大.糖醋酒液A挥发物中的对乙基苯乙酮(16.119%)和1,4-二乙酰苯(4.996%)能引起韭菜迟眼蕈蚊的电生理反应.

Abstract:In order to reveal the mechanism of the trapping effects of the sugar-acetic acid-ethanol water solutions on Bradysia odoriphaga Yang et Zhang, a field experiment was conducted for comparison of the trapping effects. The volatile component was extracted from the solutions with adapting dynamic headspace adsorptive process, and the volatile ingredient was analyzed with GC-MS, finally GC-EAD responses of four formulas of sugar-acetic acid-ethanol-water solutions (SAEW) on B.odoriphaga were tested. The trapping effects of the solution formula A (sugar:acetic acid:dehydrated alcohol:waters=3:3:1:80) is the best and has a significantly different with others (P<0.05). There are 33 volatiles in solution formula A in which the main ingredients are 7 ketones, 8 aldehydes, 2 alcohols, 12 diolefines, 3 esters, 1 phenol, but the concrete ingredients are significantly different in various SAEW formulas. 4-ethyl acetophenone and 1, 1‘-(1, 4-phenylene) bisethanone extracted from the volatiles of the solution formula A elicit the electrophysiological response of B.odoriphaga with the test of GC-EAD.

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