Abstract:A set of universal primers of geminivirus which are able to detect the components of DNA-A, DNA-B and satellite DNA of geminivirus were used in rolling circle amplification and PCR (RCA-PCR) to determine the infection of geminivirus in twelve Malvastrum coromandelianum plants samples. These 12 M.coromandelianum samples were collect from Miyi County of Sichuan Province and all of them exhibit yellow vein symptoms. To further clarify the species and mix-infection of geminiviruses, the DNA sequences were analyzed. The results showed that geminiviruses were detected in 92% samples. These samples were infected by Malvastrum yellow vein Yunnan virus (MYVYNV), Malvastrum yellow vein virus (MYVV) and Tomato yellow leaf curl China virus (TYLCCNV) and 67% samples were mix-infected by these viruses. Two types of satellite DNAβ molecules were found in 11 positive samples, and their nucleotide sequence are highly identity with either DNAβ associated with MYVYNV (MYVYNB-, 98.4% -98.7%) or DNAβ associated with MYVV (MYVB-, 98.5% -98.7%). The DNA-B and satellite DNAα molecules were not detectable from these samples. These results suggested that bego-moviruses DNA-A and their associated satellites exist as disease complex in these plants. The satellite DNAβ molecules can be associated with heterogeneous helper viruses.