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期 刊 ：植物保护学报 2009年

关键词：草莓枯萎病菌；三唑类杀菌剂；戊唑醇；敏感性；

Keywords:Fusarium oxysporum f.sp.fragariae, triazole fungicides, tebuconazole, sensitivity,

摘 要 ：为了明确山东省草莓枯萎病菌对三唑类杀菌剂的敏感性现状,从有代表性的草莓种植区采集、分离了12个草莓枯萎病菌菌株,采用菌落直径法测定其对四种三唑类杀菌剂的敏感性。结果显示,戊唑醇、烯唑醇、苯醚甲环唑和腈菌唑对12个菌株的EC₅₀值分别在0.1107~1.1670、0.1446~2.2586、0.1307~9.0317和1.1969~60.9270μg·mL^-1之间。其中菌株LY002对苯醚甲环唑和腈菌唑的敏感性较低;ZY002对苯醚甲环唑最敏感,其毒力倍数是LY002的69.10倍;TA005对腈菌唑最敏感,其毒力倍数是LY002的50.90倍;TA002对烯唑醇和TZ004对戊唑醇的敏感性均较低,而LY001对这两种药剂最敏感。结果表明,不同菌株之间对三唑类杀菌剂的敏感性差异较大。

Abstract:For confirming the sensitivity of Fusarium oxysporum f.sp.fragariae to main triazole fungicides and taking prevention and control measures, 12 strains of Fusarium oxysporum f.sp.fragariae were isolated from Shandong Province, the sensitivities of 12 strains to 4 triazole fungicides were measured by the rate of increment of colony diameter. The results showed that EC₅₀ values to the 12 strains for tebuconazole, diniconazole, difenoconazole and myclobutanil ranged from 0.1107 to 1.1670, from 0.1446 to 2.2586, from 0.1307 to 9.0317 and from 1.1969 to 60.9270 μg·mL^-1, respectively. The sensitivity of strain LY002 to difenoconazole and myclobutanil was low, strain ZY002 was the most sensitive strain to difenoconazole, its toxicity ratio was 69.10-fold compared to strain LY002, and strain TA005 was the most sensitive strain to myclobutanil, its toxicity ratio was 50.90-fold compared to strain LY002. The sensitivity of strain TA002 to diniconazole and strain TZ004 to tebuconazole were both lower, and the strain LY001 were both sensitive to these two fungicides. These results indicated that 12 strains with significantly difference in their sensitivities to four triazole fungicides.

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