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期 刊 ：植物保护学报 2010年

关键词：银胶菊花；稗草；化感作用；分离；鉴定；

Keywords:Parthenium hysterophorus flower, Echinochloa crusgalli, allelopathy, isolaton, identification,

摘 要 ：为明确银胶菊的花组织对稗草的化感作用及其活性物质成分,采用培养皿法和室内盆栽法,研究了其水浸提液的乙酸乙酯相、正丁醇相和剩余水相对稗草生长的影响。结果表明,乙酸乙酯相的化感抑制活性最高,其中,温室盆栽试验中对稗草萌发、株高、鲜重的抑制率分别为11.1%、10.2%和14.6%。采用GC-MS技术,从乙酸乙酯相较高活性组分中分离鉴定了15种主要化合物,分别为烃类及其衍生物、萘类、醇类、苯类、酸类、酮类和酯类。

Abstract:In order to ascertain the allelopathy of Parthenium hysterophorus L. flower on Echinochloa crusgalli (L.) Beauv. and allelochemicals, the allelopathic effects of ethyl acetate fraction, n-butanol fraction and residuary water fraction of P.hysterophorus flower aqueous extract on the growth of E.crusgalli were studied by the way of indoor Petri dish bioassay and indoor pot. The results in Petri dish and greenhouse showed ethyl acetate fraction had the highest bioactivity, and the inhibition of germination, plant height and fresh weight in greenhouse were 11.1%, 10.2% and 14.6% for E.crusgalli respectively at 50g/L of aqueous extract. Fifteen compounds were identified in ethyl acetate fraction by the method of GC-MS, and the constituents are mainly hydrocarbon and its derivatives, naphthalenes, alcohols, benzenes, acids, ketones and esters.

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