Abstract:In recent years, pear anthracnose caused by Colletotrichum gloeosporioides is a common disease throughout Dangshan pear-growing areas. The aim of this study was to evaluate the efficiency of Bacillus subtilis sf 628 against C.gloeosporioides by being cultured together to inhibit mycelium growth of C.gloeosporioides in vitro and sprayed on pear to control pear anthracnose. Antagonistic examination in vitro against C.gloeosporioides revealed that the inhibition rate of mycelium growth of C.gloeosporioides was 91.45%, 88.80% and 86.63%, respectively when treated with sf 628 fermented liquid, 100 fold diluted solution and 500 fold diluted solution. The inoculating experiments on pear pomes showed that the control effect reached 83.74% when fruit were first treated with sf 628 fermented liquid, 24 h later, then inoculated with C.gloeosporioides. However, when fruit were inoculated with C.gloeosporioides firstly, 24 h later, then treated with sf 628 fermented liquid, the control effect was 56.82%. In the field experiment, the control effect of sf 628 against pear anthracnose was 75.89%, 60.78% and 50.60%, respectively when treated with three concentration of sf 628 wettable powder, which was 100 fold diluted solution, 1000 fold diluted solution, and 2000 fold diluted solution. Meanwhile, the control effect of carbendazim which was common used in field was 61.10%. Therefore, B.subtilis sf 628 is a prospective antagonistic bacterium against pear anthracnose.