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期 刊 ：植物保护学报 2009年

关键词：哈茨木霉；几丁质酶；发酵条件；番茄灰霉病菌；抑制作用；

Keywords:Trichoderma harzianum, chitinase, fermentation, Botrytis cinerea, antagonistic efficiency,

摘 要 ：为了提高木霉菌株几丁质酶的产量,采用单因素和正交试验设计,优化哈茨木霉Th-30生产几丁质酶的发酵条件,并就木霉几丁质酶粗提液对番茄灰霉病菌的拮抗作用进行了探讨。结果表明,哈茨木霉Th-30以7.0g/L几丁质、2.0g/L蛋白胨、50mL/L营养盐溶液、1.0mL/L微量元素为培养基,在起始pH 5.5、温度28℃、装瓶量50mL/250mL、接种量8%的条件下,诱导96h获得的几丁质酶活性最高。哈茨木霉Th-30几丁质酶粗提液对番茄灰霉病菌有明显的拮抗作用,在稀释2倍的情况下,对番茄灰霉病菌生长的抑制率为67.61%~71.31%,对灰霉病菌孢子产生及萌发的抑制率分别为80.95%和81.35%,对病菌孢子致病性的抑制率为65.22%~70.51%。

Abstract:In order to improve the production of chitinase by Trichoderma harzianum, the medial components and culture conditions for T.harzianum Th-30 were optimized by using single factor and orthogonal tests. The results indicated that the optimal medium for inducing chitinase from T.harzianum Th-30 was composed of 7.0g/L chitin, 2.0g/L peptone, 50mL/L nutrient salt, 1.0mL/L trace element. The optimal culture conditions for inducing chitinase were as follows: 50mL medium filled in 250 mL flask with 8% inoculum size of the culture, initial pH 5.5, at 28℃, incubating for 96 hours. The chitinase extract from T.harzianum Th-30 could significantly antagonize Botrytis cinerea. When being diluted by 2 times, the inhibition rate of chitinase produced by T.harzianum Th-30 on hyphal growth of B.cinerea was 67.61%-71.31%. And it could inhibit on the generation and germination of spores of B.cinerea, by 80.95% and 81.35% respectively. The efficiency against the pathogenic ability of B.cinerea was 65.22%-70.51%.

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