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期 刊 ：植物保护学报 2010年

关键词：氯丙炔菊酯；斜纹夜蛾细胞；细胞形态；线粒体膜电位；活性氧；

Keywords:lvbingquejuzhi, Spodoptera litura (SL) cell, cell morphology, mitochondrial membrane potential, reactive oxygen species,

摘 要 ：以光活化菊酯农药新理念为指导,设计合成了氯丙炔菊酯的二炔衍生物(JZB),并以胺菊酯、溴氰菊酯、联苯菊酯和氯丙炔菊酯为对照药剂,采用MTT法、Giemsa染色法和流式细胞术研究JZB对斜纹夜蛾Spodoptera litura (SL)细胞的光活化毒力和毒性机制。结果表明,500μg/mL处理SL细胞后24h,氯丙炔菊酯及JZB均表现出良好的抑制活性,抑制率分别达89.79%和92.95%,而胺菊酯、溴氰菊酯和联苯菊酯的抑制率均低于21%;氯丙炔菊酯及JZB对SL细胞形态影响明显,50μg/mL处理后24h,细胞密度降低,90%以上的细胞收缩变圆,无法正常贴壁,而500μg/mL溴氰菊酯处理后24h,70%以上细胞贴壁状态良好;经50μg/mL氯丙炔菊酯及JZB处理后,细胞线粒体膜电位较对照分别降低了35.43%和45.55%,活性氧较对照分别升高212.09%和311.60%。

Abstract:The two-acetylene derivative of lvbingquejuzhi (JZB) was synthesized here for the hypothesis of new photoactivated pyrethroid insecticides. The MTT assay, Giemsa dying method and flow cytometry were chosen to evaluate the photoactivated cytotoxicity of JZB on Spodoptera litura (SL) cell, compared with that of tetramethrin, deltamethrin, bifenthrin and lvbingquejuzhi. The results showed that the inhibition rates of 500μg/mL tetramethrin, deltamethrin and bifenthrin were lower than 21%. But 500μg/mL lvbingquejuzhi and JZB possessed high cytotoxicity, the inhibition rates were 89.79% and 92.95%, respectively, and cell density was significantly lower, above 90% cells shrank and could not attach to the bottom. But above 70% cells treated with 500μg/mL deltamethrin could attach to the bottom well. When SL cells exposed to 50μg/mL lvbingquejuzhi and JZB for 24 hours, MMP respectively decreased by 35.43% and 45.55% compared with the control group, and ROS of SL cells increased by 212.09% and 311.60%.

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