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期 刊 ：植物保护学报 2010年

关键词：辣椒碱；斜纹夜蛾细胞；细胞周期；细胞凋亡；流式细胞仪；

Keywords:capsaicin, Spodoptera litura cell, cell cycle, apoptosis, flow cytometry,

摘 要 ：采用3-(4,5-二甲基-2-噻唑)-2,5-二苯基溴化四唑(MTT)检测法、荧光显微镜细胞形态观察法和流式细胞仪(flow cytometry, FCM)技术,研究了辣椒碱对离体培养斜纹夜蛾Spodoptera litura (SL)细胞增殖及凋亡的影响。结果表明:辣椒碱对SL细胞24h的IC₅₀值为46.89μg/mL,与鱼藤酮差异不显著。40μg/mL辣椒碱处理SL细胞24h后,细胞形态明显不规则,呈现出 凋亡的典型症状。 10、 20和40 μg/mL辣椒碱处理SL细胞48 h后, 细胞早期凋亡率分别为13.56%、18.61%和3.81%,而晚期凋亡和坏死率分别为23.78%、34.28%和61.72%。辣椒碱处理细胞48h后,细胞周期变化明显,细胞生长阻滞于G2/M期。表明辣椒碱对斜纹夜蛾细胞具有良好的抑制作用,能致细胞凋亡并有一定的浓度依赖性。

Abstract:MTT method, fluorescence microscope and flow cytometry (FCM) were used to explore the effects of capsaicin on proliferation and apoptosis of Spodoptera litura (SL) cells. The results showed that the IC₅₀ value of capsaicin to SL cells for 24h was 46.89μg/mL, and the difference was not significant when compared with rotenone. After SL cells were treated by 40μg/mL capsaicin for 24h, the cell morphology changed obviously and typical symptom of apoptosis was detected. After SL cells were treated by 10, 20 and 40μg/mL capsaicin for 48h, the rates of early apoptosis were 13.56%, 18.61% and 3.81%, respectively, while the late apoptosis were 23.78%, 34.28% and 61.72%, respectively. The change of cell cycle was obvious and cell cycle arrested in the G2/M phase after cells were treated by capsaicin for 48h. It is tentatively concluded that the inhibitory effect of capsaicin to SL cells was good and capsaicin possessed the concentration dependence induction of apoptosis to SL cells.

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