Abstract:The purpose of the studies was to sieve a good method to preserve the viability of fresh pure isolates of Blumeria graminis (DC) Speer f. sp. tritici Marchal. The efficacy comparisons among 5 leaf-freshening formulations selected from preliminary experiments were performed with three different techniques, i.e. detached-leaf-culture with filter paper,detached-leaf-culture with agar and test tube growing seedlings with soil. The results showed that among 5 formulations the most potent one was Shenbao 6 and its optimum concentration was 40μg/mL, the green color ratio of wheat leaves was maintained over 90% on the 25th day. Furthermore, tests were also conducted to select ideal preservation environment conditions, the result indicating that at 4℃in the refrigerator, either the technique to keep detached infected leaves on Shenbao 6 wetted filter paper in covered petri dish or the one to maintain the leaves in Shenbao 6 agar in sealed bottles was ideal for holding the viability of the spores, which could maintain green color ratio of the leaves about 78% and 68% and the spore germination rate about 26.7% and 26.8%, respectively, until the 40th day.