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期 刊 ：植物保护学报 2005年

关键词：紫外保护剂；昆虫病原真菌；分生孢子保护；

Keywords:UV protectant, entomopathogenic fungi, conidial protection,

摘 要 ：在室内检测了17种紫外保护剂和4种组合对3种昆虫病原真菌即球孢白僵菌Beauveria bassiana、金龟子绿僵菌Metarhizium anisopliae、蜡蚧轮枝孢Verticillium lacani分生孢子的保护作用。结果表明,苯并三唑类2^#的紫外保护作用最好,用紫外杀菌灯照射30 min,苯并三唑类2^#对3种菌株孢子的保护效率均达90%以上;苯并三唑类3^#、苯甲酮类2^#、荧光黄钠、刚果红的保护效率可达60%。组合使用紫外保护剂并不能增强保护效果。选用苯并三唑类2^#时,适宜的溶剂是花生油加正己烷(1 ∶ 1, v/v),使用浓度应大于0.75%。用254、312、365 nm波长紫外灯照射40 min内,1%苯并三唑类2^#对3种菌株孢子的保护效率可保持90%,照射180 min后,保护效率在56％~77%。

Abstract:The UV-screen effect of 17 UV-protectants and 4 combinations for conidia of Beauveria bassiana,Metarhizium anisopliae and Verticillium lacanii was inspected in laboratory.UVP-2 (benzotriazole 2^#) was confirmed to be the most effective protectant.Protective efficiency of UVP-2 to spores of entomopathogenic fungi was over 90%, and other 4 UVPs (benzotriazole 3^#, oxybenzone 2^#, sodium uranine and Congo red) were about 60% after 30 min exposure of spores to UV. Mixture of UVPs did not show higher effectiveness compared to single UVP. The mixture of peanut oil and n-hexane was suitable solute for UVP-2.The effective concentration of UVP-2 should be higher than 0.75% for the protection of fungal conidia. After 40 min and 180 min exposure of fungal conidia to UV light (at λ_{254 nm}, λ_{312 nm} or λ_{365 nm} ), the protective efficiency of UVP-2 could be increased over 90% and 56%-77%, respectively.

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