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作 者 ：陈磊, 朱月林, 杨立飞, 王聪

期 刊 ：植物营养与肥料学报 2010年 16卷 3期 页码：768-772

关键词：氮素形态；菜用大豆；抗氧化酶；膜脂过氧化；

Keywords:nitrogen forms, vegetable soybean, antioxidant enzyme activity, membrane lipid peroxidation,

摘 要 ：通过蛭石盆栽试验，研究了氮素不同形态配比对菜用大豆［Glycine max (L.) Merr.］品种“理想95-1”生长、种子抗氧化酶活性及活性氧代谢的影响。结果表明，营养液中适宜的硝铵比（75∶25）有利于菜用大豆的生长发育，植株具有最大生物量； 在高比例的硝态氮（100%）和铵态氮（75%）处理下，植株的干重、鲜重及产量均显著降低，以硝铵比为25∶75处理下尤为显著。在适宜的硝铵比（75∶25和50∶50）处理下，菜用大豆种子具有较低的抗氧化酶活性，活性氧代谢产物O₂^-、过氧化氢（H_2O2）和膜脂过氧化产物丙二醛（MDA）含量也较低，表明植株受到的氧化胁迫程度较低； 而在硝铵比为25∶75处理中，抗氧化酶活性最高，O₂^-生成速率、H_2O2和MDA含量也最高，表明过多的铵态氮对细胞膜造成了伤害，所受的氧化损伤程度较重。

Abstract:Using the vermiculite culture, the effects of ratios of NO^-₃-N and NH⁺₄-N on plant growth, seed antioxidant enzyme activities and reactive oxygen metabolism of vegetable soybean ［Glycine max (L.) Merr. cv. Li-xiang 95-1］ were studied. The results show that the appropriate ratio of NO^-₃-N and NH⁺₄-N is about 75∶25 which is beneficial to the growth and development of the soybean, and produces the maximum plant biomass. Under the treatment of excessive NO^-₃ (100%) or NH⁺₄ (75%), both biomass production and yields are decreased obviously, especially for the NH⁺₄ (75%) treatment. In the NO^-₃∶NH⁺₄ treatments of 75∶25 and 50∶50, the activities of antioxidant enzymes are low, and the O₂^ producing rate, hydrogen peroxide (H_2O2) and malondiadehyde (MDA) contents are also low, therefore the degree of oxidative stress is comparatively low. However, under the NO^-₃∶NH⁺₄ treatment of 25∶75, the antioxidant enzyme activities, the O₂^ producing rate, H_2O2 and MDA contents reach to their highest values. These results indicate that excessive NH⁺₄ is harmful to cell membrane integrity, resulting in severe degree of oxidative damage in the seeds of vegetable soybean.

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