Abstract:More than 20 field experiments designed with the “3414” plan were carried out in Huxian County and Zhouzhi County, Guanzhong Plain, Shaanxi during 2008 and 2009 to evaluate the NO3-N accumulation in soil profiles after harvesting winter wheat; and its relationship with the apparent N budget in soil was also studied. The results show the amounts of NO3-N accumulation in the soil profiles and their extents to leach into the deeper soil layers are increased significantly as the nitrogen fertilizer usage increased. When the N fertilizer rate reaches to 180–240 kg/ha, positive budget of nitrogen in soil is observed in some experiment sites, and when N fertilizer rate reaches to the 270–360 kg/ha, positive budget of nitrogen in soil is observed in all experiment sites. There is a positive correlation between the apparent balances of soil N and the accumulation of NO3-N in soil profiles (0–2 m). This indicates the amount of NO3-N in the soil profile is mainly determined by the apparent N budget balances in soil. The NO3-N accumulation in the soil profile is increased about 62.5 kg/ha when N balance in soil is at 100 kg/ha.