---

期 刊 ：植物保护学报 2000年

关键词：苏云金杆菌；转基因水稻；螟虫；抗性；离体叶片测定；

Keywords:Bacillus thuringiensis, transgenic rice, rice stem borers, resistance, de-tached-leaf bioassay,

摘 要 ：提出了转苏云金杆菌ｃｒｙｌＡｂ基因水稻抗螟性的快速离体叶片测定法，并用该方法评价了转ｃｒｙ１／Ａｂ基因水稻对二化螟、三化螟和大螟的抗性程度。结果表明，３种螟虫蚁螟取食纯合株系ＰＲ１６及分离株系ＰＲ１８、ＰＲ５７或ＰＲ５８中抗性植株中片后，食叶量与对照和分离株系中的非抗性植株相比极显著下降，接虫后第４ｄ的幼虫死亡率高达１００％。该方法评价转ｃｒｙ１／Ａｂ基因水稻对３种螟虫的抗性结果与活化成株测定法评

Abstract:The "detached-leaf bioassay", a rapid and easy method for determining the resistance of Bt (cry1Ab gene of Bacillus thuringiensis Berliner) transgenic rice to three stem borers, namely, striped stem borer Chilo suppressalis (Walker), yellow stem borer Sci

---