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作 者 ：吴观以;李慧荃;李元芳;孙建光;姚允寅;陈明;张希忠

期 刊 ：植物营养与肥料学报 1995年 1卷 2期 页码：61-64

关键词：胡萝卜离体根；类根瘤；4-D；15N2还原法；

Keywords:Organculturedcarrotroots.Para-nodules, 2, 4-Dichlorophenoxyaceticacid, 15N2reductionmethod.,

摘 要 ：用低浓度的2,4-D处理胡萝卜离体根诱导结瘤,并接种固氮螺菌(Azospirillum.brasilense),用¹⁵N₂还原法测定结果证明,胡萝卜离体类根瘤能固定¹⁵N₂。电镜切片观察发现,类根瘤内有菌体存在,菌体进入细胞间隙中比进入细胞内多。

Abstract:Nitrogen fixation of para-nodules of organ cultured carrot roots was assured with ¹⁵N₂ reduction method after the par a-nodules of carrot roots were induced with low concentration 2,4-D and the inoculation of Azospirillum brasilense. More bacteria in intercellular spaces and less in cells of para-nodules were also observed under electronic microscope.

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