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作 者 ：胡小加;张学江;黄沁洁;江木兰;EdwardCCocking;CordonSWebster

期 刊 ：植物营养与肥料学报 1997年 3卷 3期 页码：275-279

关键词：果胶细菌；根瘤菌；油菜；类根瘤；

摘 要 ：果胶细菌处理油菜幼根（2天苗龄、根长3cm），同时接种紫云英根瘤菌突变菌株（Rhizobiumhuakuii），诱发类根瘤的结瘤率为96.80％。从这些类根瘤中分离的菌株能回接到原寄主。类根瘤的电镜观察可以明显地见到细胞内充满了细菌。乙炔还原法测定的固氨酶活性（C₂H₄）为76.80nmol／（plant·h）。

Abstract:Nodular structures have been induced on oilseed rape root by treating the 3cm length roots of 2-d seedlings with a pectobacterium, followed by inoculation with a mutunt strain of Rhizobium astraglus, the nodulation rate is 96. 80%. The strains were isolated from nodules of oilseed rape and successfully reinoculated to original host.Transverse section of para-nodules showed that cells of nodule were full of bacteria.The activity of the nodules was determined by acetylene reduction method (C₂H₄,76. 80 nmol·plant^-1·h^-1).

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