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作 者 ：肖焱波1;3;李隆2;张福锁2

期 刊 ：植物营养与肥料学报 2006年 12卷 1期 页码：89-96

关键词：根瘤菌接种；固氮；根系分隔；小麦；蚕豆；

Keywords:inoculation, N fixation, root barriers, wheat, faba bean,

摘 要 ：采用不同根系分隔方式(小麦与蚕豆根系完全分隔、尼龙网分隔和不分隔)研究了间作蚕豆接种根瘤菌菌株NM353,在施氮和不施氮情况下,蚕豆和小麦的生长和养分吸收状况。结果表明,无论何种分隔方式和施氮与否,接种根瘤菌菌株NM353对蚕豆的生长都好于不接种;同时,间作小麦也通过根系的相互作用受益。在施氮情况下,接种与不接种处理相比,蚕豆生物量在三种分隔方式中依次增加了15%、16%和5%;吸氮量依次增加了17%、9%和12%;而根系不分隔并接种根瘤菌NM353还改善间作小麦的生长,其生物量、吸氮量比不接种时分别增加了13%和22%。因此,小麦/蚕豆间作中通过选择合适的菌株进行接种,并配合适当的氮肥施用,可以充分发挥豆科生物固氮作用,并改善与之间作的作物氮素营养。

Abstract:The purpose of the experiments reported here was to determine the effects of inoculating rhizobia strain NM353 on the growth and N use between intercropped faba bean(Vicia faba L.cv.Linxia Dacandou) and wheat(Triticum aestivum L.cv.Long 17),and to quantify the contribution of N fixation of faba bean to the companion crop wheat.The root barriers were used to assess the contribution of interspecific root interaction to crops‘ growth via pot experiment.Accordingly,the pots at the volume at(π×7²×15)cm³ were separated into two compartments,either by a solid barrier(plastic sheet) to prevent root contact and N movement,by nylon meshes(30 μm) to prevent root contact,but allow N exchange, or no root barrier between the compartments to allow root interaction.Thereafter,faba bean was inoculated with rhizobia strain NM353 either under the condition of N application and non-N application in three different root barriers.The results showed that wheat growth was enhanced without root barrier no matter with N or without N,leading to higher biomass and N acquisition than those with both solid barrier and mesh barrier.Root interaction did improve the growth of wheat,and so did N application.Faba bean growth was not affected by root barriers with NM353 inoculation but affected without NM353 inoculation.Rhizobia strain NM353 inoculation did improve the growth of faba bean with(solid) barrier,while N reduces the growth of faba bean.Obviously,the inoculation effects on faba bean in intercropping differed in root barriers and N application,but the growth of faba bean was enhanced on the average at 12% compared with that without innoculation.The biomass of faba bean was increased 15%,16% and 5%,respectively with respect to the three root barriers under N application,and accordingly the N acquisition of faba bean was increased at 17%,9% and 12%,respectively.Only when interspecific roots interaction occurred could wheat benefit from inoculating rhizobia strain NM353 on faba bean.The growth of wheat without root barrier was improved by inoculating NM353 either with N or not,leading to higher biomass and N acquisition.Under N application,the biomass of wheat was enhanced by 13% compared with that without inoculation,and N acquisition by 22%.Thereby,the feasible way existed in inoculating appropriate rhizobium strain for intercropping that would improve N nutrition of both cereal and legumes.

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