---

期 刊 ：植物保护学报 2003年

关键词：葡萄；卷叶病毒；RT-PCR检测技术；逆转录-聚合酶链式反应；

Keywords:RT-PCR, GLRaV, cloning sequencing,

摘 要 ：为调查葡萄植株携带葡萄卷叶病毒(GLRaV)的情况，以带毒和非带毒指示植物“品丽珠”为试验材料提取总RNA，并以此总RNA为模板进行RT—PCR扩增，建立了GLRaV的RT—PCR检测体系，并对GLRaV cDNA序列进行测定，结果成功地检测到了GLRaV。经多次试验证明，该检测结果准确可靠，可以用于GLRaV的检测。

Abstract:The virus indicator plants Cabernet Franc were chosen as experimental material to establish the RT-PCR detection system for GLRaV. After the total RNA was extracted, the GLRaV was deteced by RT-PCR. The amplification of 300 bp cDNA sequence from GLRaV-inf

---