Abstract:The trace of red O-phenanthroline Fe indicates: Fe2+ was absorbed by roots and transported to stem, main vein of leaves when iron fertilizer was root inserted.Fe2+ transports along xylem near cambium. The speed of transportation reached dozens of centimeters per hour. The trace of 59Fe with Malus micromalus cultured in nutrient solution under the controlled conditions indicate: distribution of 59Fe in cutting roots and leaves were 18.1% and 70 9%, respectively. The trace of 59Fe with Malus micromalus indicate: the ratios of iron distributed in leaves by cutting one, two, three roots were 57.9%, 63.6 %and 68.0%, respectively.